Antibodies for Neuroscience Research

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Western blot image of activated mouse recombinant LIMK1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-LIMK1 (C-term.) (lanes 1 & 2) and anti-LIMK1 (Thr-508) (lanes 3 & 4).
Native western blot image of human laminin isoforms: laminin 521 (α5β2γ1), laminin 121 (α1β2γ1), laminin 221 (α2β2γ1), laminin 332 (α3β3γ2), laminin 511 (α5β1γ1), laminin 411 (α4β1γ1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin β2/γ1 subunit (LM0461) at 1:1000.Immunocytochemical labeling of laminin β2/γ1 subunits in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Laminin β2/γ1 subunits (LM0461). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight® 594.Representative Standard Curve using mouse monoclonal antiL1CAM (LM0231) for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blot analysis of PC12 cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-JNK1 (lanes 1 & 2) or anti-JNK1 (T183/Y185) (lanes 3 & 4).Immunocytochemical labeling of JNK in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with mouse monoclonal JNK (C-terminal region) (Left) or mouse monoclonal JNK (Thr-183/Tyr-185) (Right). The antibodies were detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of Hsp60 in mitochondria in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal Hsp60 (HM4381). The antibody was detected using goat anti-mouse DyLight® 488.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
Western blot analysis of a human recombinant GSK3β N-terminal fragment (lanes 1 & 2) and endogenous GSK3β expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3β at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).Immunocytochemical labeling of GSK3β in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK3β (GM1311) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.
Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells
were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight® 594.

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