Our mouse Shank1 FL490 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N22/21. It detects human, mouse, and rat Shank1 and is purified by Protein A chromatography. Works in IHC, ICC.
SH3 and multiple ankyrin repeat domains protein 1, or shank 1 is a member of the shank family. Shank 1 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Unlike other shank family proteins, shank 1 is found in only in brain in the neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum, amygdala, substantia nigra and thalamus. Cellularly, shank 1 can be found in cytoplasm, cell junctions, postsynaptic cell membrane and postsynaptic density. Many isoforms of shank 1 exist, and some are expressed during development, while others are expressed in mature tissue. Shank 1 acts as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 1 may play a role in synapse formation and dendritic spine maturation. Mutations in this gene have been associated with autism spectrm disorder and diabetic encephalopathy.
Purified by Protein A chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Monoclonal
N22/21
IgG1
ICC, IHC
Mouse
Shank1
190-220 kDa (alternative splice variants)
Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1 (accession number Q9WV48) produced recombinantly in E. Coli
Rat
Human, Mouse, Rat
AB_2939268
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL490 Ex: 491 nm, Em: 515 nm
No cross-reactivity against Shank2 or Shank3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from date of receipt
GKAP/SAPAP-interacting protein; SH3 and multiple ankyrin repeat domains protein 1; Shank1a; Shank1; Sstrip; SAPAP-interacting protein synamon; SPANK-1; SH3/ankyrin domain gene 1; Somatostatin receptor-interacting protein; SSTR-interacting protein; Synamon
Q9WV48
UniProt Summary: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function.
