Anti-Shank1 Antibody (N22/21)

Name: Anti-Shank1 Antibody
Brand: NeuroMab
SKU: 75-064
Price: 308.0 USD
Availability: InStock

from NeuroMab Datasheet

8 Citations

Available Conjugates

490

550

594

650

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

N22/21

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Purified by Protein A chromatography

Gene Name

Shank1

Molecular Weight

190-220 kDa (alternative splice variants)

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Transfected cell immunofluorescence staining N22/21=green, rabbit anti-Shank1=red, DAPI=blue.

Adult rat brain membrane immunoblot.

Volume: 100 µL

Variant

Price:

$308

Quantity:

Bulk Order Anti-Shank1 Antibody

Product Details

Target

Shank1

Target Description

SH3 and multiple ankyrin repeat domains protein 1, or shank 1 is a member of the shank family. Shank 1 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Unlike other shank family proteins, shank 1 is found in only in brain in the neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum, amygdala, substantia nigra and thalamus. Cellularly, shank 1 can be found in cytoplasm, cell junctions, postsynaptic cell membrane and postsynaptic density. Many isoforms of shank 1 exist, and some are expressed during development, while others are expressed in mature tissue. Shank 1 acts as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 1 may play a role in synapse formation and dendritic spine maturation. Mutations in this gene have been associated with autism spectrm disorder and diabetic encephalopathy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N22/21

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Shank1

Molecular Weight

190-220 kDa (alternative splice variants)

Antigen

Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1 (accession number Q9WV48) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2270283

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

No cross-reactivity against Shank2 or Shank3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon)

UniProt Details

UniProt (Human): Q9Y566
UniProt (Immunogen Species): Q9WV48

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34852231	Not listed	Lautz, J., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell reports, .
23038781	Not listed	Catarino, T., et al. 2013. Regulation of synapse composition by protein acetylation: the role of acetylated cortactin. Journal of cell science, 149-162.

Immunocytochemistry: Rat
PMID	Dilution	Publication
26547831	1:400	MacGillavry, H., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. The European journal of neuroscience, 179-193.
21795692	Not listed	Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34839-34850.

Western Blot: Mouse
PMID	Dilution	Publication
26527743	1:1000	Schreiber, J., et al. 2015. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic γ-actin levels. The Journal of cell biology, 569-586.
24153177	Not listed	Han, K., et al. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 72-77.

Western Blot: Rat
PMID	Dilution	Publication
26215919	1:20 (supe)	Farley, M., et al. 2015. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience, 286-301.
21795692	Not listed	Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34839-34850.

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Anti-Shank1 Antibody (N22/21)

Available Conjugates

Anti-Shank1 Antibody
SKU: 75-064

Bulk Order Anti-Shank1 Antibody

Product Details

Product Specific References for Applications and Species

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Anti-Shank1 Antibody (N22/21)

Available Conjugates

Anti-Shank1 Antibody SKU: 75-064

Bulk Order Anti-Shank1 Antibody

Product Details

Product Specific References for Applications and Species

Anti-Shank1 Antibody
SKU: 75-064