SH3 and multiple ankyrin repeat domains protein 1, or shank 1 is a member of the shank family. Shank 1 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Unlike other shank family proteins, shank 1 is found in only in brain in the neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum, amygdala, substantia nigra and thalamus. Cellularly, shank 1 can be found in cytoplasm, cell junctions, postsynaptic cell membrane and postsynaptic density. Many isoforms of shank 1 exist, and some are expressed during development, while others are expressed in mature tissue. Shank 1 acts as a scaffold protein anchoring and connecting membrane bound receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 1 may play a role in synapse formation and dendritic spine maturation. Mutations in this gene have been associated with autism spectrm disorder and diabetic encephalopathy.
Purified by Protein A chromatography
ICC, IHC, WB
190-220 kDa (alternative splice variants)
Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1 (accession number Q9WV48) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
No cross-reactivity against Shank2 or Shank3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from date of receipt
Shipped on ice packs
SH3 and multiple ankyrin repeat domains protein 1 (Shank1) (GKAP/SAPAP-interacting protein) (SPANK-1) (Somatostatin receptor-interacting protein) (SSTR-interacting protein) (SSTRIP) (Synamon)