Anti-PICK1 Antibody (L20/8)

Name: Anti-PICK1 Antibody
Brand: NeuroMab
SKU: 75-040
Price: 337.0 USD
Availability: InStock

Our mouse PICK1 monoclonal antibody from NeuroMab is produced in-house from clone L20/8. It detects human, mouse, and rat PICK1 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

$Immunoblot against membrane fractions from adult rat brain (RBM) and hippocampi (MHM) from wild-type (WT) and PICK1 knockout (KO) mice probed with L20/8 TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).$

Adult rat hippocampus immunohistochemistry.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe.$

Immunoblot against membrane fractions from adult rat brain (RBM) and hippocampi (MHM) from wild-type (WT) and PICK1 knockout (KO) mice probed with L20/8 TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, HHMI).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-040

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PICK1

Scientific Background

PRKCA-binding protein or PICK1 scaffold is encoded by the gene PICK1. PICK1 is a scaffold protein which acts as an adaptor protein that binds and organizes the location of membrane proteins. Functions to cluster receptors and plays a role in synaptic plasticity. PICK1 is ubiguitously expressed and found in high levels in brain and testis. Cellularly, PICK1 is found at perinuclear region, mitochondria and synapses. Diseases associated with PICK1 include Spermatogenic Failure and Depression.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L20/8

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

PICK1 PRKCABP

Molecular Weight

45 kDa

Immunogen

Fusion protein amino acids 10-130 of human PICK1 (accession number AAG48152) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2164544

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

PICK; PRKCA-binding protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein

UniProt ID

Q9NRD5
UniProt Summary: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

NCBI Gene ID

9463

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Anti-PICK1 Antibody (L20/8)

SKU: 75-040

Product Details

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