Anti-MPP8 Antibody (N119/44)

Name: Anti-MPP8 Antibody
Brand: NeuroMab
SKU: 75-154
Price: 337.0 USD
Availability: InStock

Our mouse MPP8 monoclonal antibody from NeuroMab is produced in-house from clone N119/44. It detects human MPP8 and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transfected with GFP-tagged MPP8 and untagged Kv2.1 plasmids and probed with N119/44 (left panel) and K89/34 (right panel) TC supe.

Transfected cell immunofluorescence: COS cells expressing GFP-tagged human MPP8. Red = N119/44, Green = GFP, Blue = Hoechst nuclear stain.

Transfected cell immunoblot: extracts of COS cells transfected with GFP-tagged MPP8 and untagged Kv2.1 plasmids and probed with N119/44 (left panel) and K89/34 (right panel) TC supe.

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Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-154

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MPP8

Scientific Background

M-Phase Phosphoprotein 8 is encoded by the gene MPHOSPH8. MPHOSPH8 is a heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. MPHOSPH8 is expressed in the brain. Diseases associated with MPHOSPH8 include Charcot-Marie-Tooth Disease.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N119/44

Isotype

IgG2b

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

MPHOSPH8 MPP8

Molecular Weight

100 kDa

Immunogen

Fusion protein amino acids 50-120 (Chromo domain) of human MPP8 (accession number Q99549) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_2146181

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.80

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

HSMPP8; M-phase phosphoprotein 8; mpp8; Two hybrid-associated protein 3 with RanBPM; Twa3; M-phase phosphoprotein; mpp

UniProt ID

Q99549
UniProt Summary: Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. As part of the HUSH complex, promotes epigenetic repression of mobile genetic elements, such as retroviruses and transposable elements: the HUSH complex mainly represses LINE-1 (L1) retrotransposons that are still capable of transposition. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression. MPHOSPH8 mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2, a chromatin remodeler that compacts chromatin. The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed. As part of the HUSH2 complex, promotes epigenetic repression of interferon-stimulated genes. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Mediates silencing of E-cadherin (CDH1) and protocadherin genes in the nervous system.

NCBI Gene ID

54737

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Anti-MPP8 Antibody (N119/44)

SKU: 75-154

Product Details

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