Anti-DNMT3L Antibody (N117/9)

Name: Anti-DNMT3L Antibody
Brand: NeuroMab
SKU: 75-153
Price: 337.0 USD
Availability: InStock

Our mouse DNMT3L monoclonal antibody from NeuroMab is produced in-house from clone N117/9. It detects human DNMT3L and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L, DNMT3A and DNMT3B plasmids and probed with N117/9 TC supe (left) and mouse anti-Flag (right).

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L, DNMT3A and DNMT3B plasmids and probed with N117/9 TC supe (left) and mouse anti-Flag (right).

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-153

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

DNMT3L

Scientific Background

DNA (cytosine-5)-methyltransferase 3-like or DNMT3L is encoded by the gene DNMT3L. DNMT3l is a nuclear, catalytically inactive, regulatory factor of DNA methyltransferases. DNMT3L mediates transcriptional repression through interaction with histone deacetylase 1. It is thought to be required for the establishment of maternal genomic imprints. DNMT3L is expressed in several tissues including testis, ovary, and thymus. Diseases associated with DNMT3L include Testicular Spermatocytic Seminoma and Immunodeficiency-Centromeric Instability-Facial Anomalies Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N117/9

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

DNMT3L

Molecular Weight

45 kDa

Immunogen

Fusion protein amino acids 1-387 (full-length) of human DNMT3L (accession number BAA95556) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_10673565

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.79

Conjugate

Unconjugated

Specificity

No cross-reactivity against DNMT3A or DNMT3B

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

DNA (cytosine-5-methyltransferase 3-like; DNA; cytosine-5-methyltransferase 3-like protein; cytosine-5-methyltransferase 3-like; human cytosine-5-methyltransferase 3-like protein

UniProt ID

BAA95556
UniProt Summary: Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression.

NCBI Gene ID

29947

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Anti-DNMT3L Antibody (N117/9)

SKU: 75-153

Product Details

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