Anti-MMACHC Antibody (N230/21)

Name: Anti-MMACHC Antibody
Brand: NeuroMab
SKU: 75-239
Price: 337.0 USD
Availability: InStock

Our mouse MMACHC monoclonal antibody from NeuroMab is produced in-house from clone N230/21. It detects human and mouse MMACHC and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.

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Species Reactivity

Human, Mouse

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-239

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MMACHC

Scientific Background

Metabolism of Cobalamin Associated C is encoded by the gene MMACHC. MMACHC is a member of the MMACHC family. MMACHC may have a role in the binding and intracellular trafficking of of cobalamin. MMACHC is widely expressed with higher levels in the liver, spleen, lymph nodes, thymus, and bone marrow. Diseases associated with MMACHC include Methylmalonic Aciduria and Homocystinuria, Cblc Type and Methylmalonic Acidemia with Homocystinuria.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N230/21

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

MMACHC

Molecular Weight

32 kDa

Immunogen

Fusion protein amino acids 1-282 (full-length) of human MMACHC (accession number Q9Y4U1) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_10673577

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

cblC; Methylmalonic aciduria and homocystinuria type C protein; cyanocobalamin reductase (cyanide-eliminating; EC 1.16.1.-; Cyanocobalamin reductase; cyanide-eliminating

UniProt ID

Q9Y4U1
UniProt Summary: Cobalamin (vitamin B12) cytosolic chaperone that catalyzes the reductive decyanation of cyanocob(III)alamin (cyanocobalamin, CNCbl) to yield cob(II)alamin and cyanide, using FAD or FMN as cofactors and NADPH as cosubstrate. Cyanocobalamin constitutes the inactive form of vitamin B12 introduced from the diet, and is converted into the active cofactors methylcobalamin (MeCbl) involved in methionine biosynthesis, and 5'-deoxyadenosylcobalamin (AdoCbl) involved in the TCA cycle. Forms a complex with the lysosomal transporter ABCD4 and its chaperone LMBRD1, to transport cobalamin across the lysosomal membrane into the cytosol. The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine synthase reductase) and MTR (methionine synthase) which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine. Also acts as a glutathione transferase by catalyzing the dealkylation of the alkylcob(III)alamins MeCbl and AdoCbl, using the thiolate of glutathione for nucleophilic displacement to generate cob(I)alamin and the corresponding glutathione thioether. The conversion of incoming MeCbl or AdoCbl into a common intermediate cob(I)alamin is necessary to meet the cellular needs for both cofactors. Cysteine and homocysteine cannot substitute for glutathione in this reaction.

NCBI Gene ID

25974

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Anti-MMACHC Antibody (N230/21)

SKU: 75-239

Product Details

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