Anti-GAD67 Antibody (L127/8)

Name: Anti-GAD67 Antibody
Brand: NeuroMab
SKU: 75-507
Price: 337.0 USD
Availability: InStock

Our mouse GAD67 monoclonal antibody from NeuroMab is produced in-house from clone L127/8. It detects human, mouse, and rat GAD67 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA, AT.

Array tomography immunofluorescence of an Lowicrylembedded 70 nm section from adult mouse neocortex with L127/8 (green), guinea pig polyclonal GABA (Millipore AB175, magenta) and DAPI (blue). Image courtesy of Kristina Micheva (Stanford).

Western blotting of rat brain homogenate (10 ug/lane) with anti-GAD65/67 (L127/8) at 1:100 dilution and detected with anti-mouse HRP.

Array tomography immunofluorescence of an Lowicrylembedded 70 nm section from adult mouse neocortex with L127/8 (green), guinea pig polyclonal GABA (Millipore AB175, magenta) and DAPI (blue). Image courtesy of Kristina Micheva (Stanford).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-507

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GAD67

Scientific Background

Glutamate decarboxylase 1, GAD67 or GAD1 is a member of the group II decarboxylase family and catalyzes the production of GABA from L-glutamic acid. GAD67 is expressed in brain (throughout the cell), in insulin producing ßcells of the pancreas, and several non-neuronal tissues during development. Alterations in GAD67 have been shown to lead to pyridoxine dependency with seizures. Other disorders associated with GAD67 are Cerebral Palsy, Spastic Quadriplegic, 1 and Inherited Congenital Spastic Tetraplegia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

L127/8

Isotype

IgG2b

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

GAD1, GAD, GAD67

Molecular Weight

70 kDa

Immunogen

Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2756509

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Cross-reacts with GAD25 Does not cross-react with GAD44 or GAD65/GAD2

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

CPSQ1; GAD67; SCP; glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD-67; glutamate decarboxylase 1 (brain; 67kDa; glutamate decarboxylase 67 kDa isoform; GAD1; GAD and DCE1

UniProt ID

Q99259
UniProt Summary: Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.

NCBI Gene ID

2571

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Anti-GAD67 Antibody (L127/8)

SKU: 75-507

Product Details

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