Our Anti-GABA(A)R, Beta1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N96/55. It detects human and mouse GABA(A)R, Beta1, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Gamma-aminobutyric acid receptor subunit beta-1 or -GABA AR-Beta1 is encoded by the gene GABRB1 GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-Beta1 is primarily expressed in brain at the cell junction, synapse,and the postsynaptic cell membrane. Diseases associated with GABRB1 include early infantile epileptic encephalopathy 43, neurotic disorder, schizophrenia and autism.
Purified by Protein A chromatography
Fusion protein amino acids 327-450 of mouse GABA-A-R-Beta1 (accession number P50571) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL550 Ex: 550 nm, Em: 575 nm
No cross-reactivity against GABA-A-R-Beta2 or -Beta3
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.