Anti-Frataxin Antibody FL490 Conjugate (N191/7)

Name: Anti-Frataxin Antibody FL490 Conjugate
Brand: NeuroMab
SKU: 75-207-FL490
Price: 486.0 USD
Availability: InStock

Our mouse Frataxin FL490 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N191/7. It detects human Frataxin and is purified by Protein A chromatography. Works in ICC.

Anti-Frataxin Antibody FL490 Conjugate

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Species Reactivity

Human, Mouse

Applications

ICC

Host Species

Mouse

Conjugates and Formulations

Unconjugated

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-207-FL490

Volume: 200 µL

Variant

Price:

$486.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Frataxin

Scientific Background

Frataxin is a highly conserved protein found in mitochondria. Frataxin is found many tissues including heart, spinal cord, liver, pancreas, dermal fibroblasts, peripheral blood lymphocytes and skeletal muscles. Frataxin functions as an iron chaperone involved in iron-sulfur biogenesis and heme biosynthesis and plays a role in iron homeostasis. Mutations in the Frataxin gene can result in Friederich ataxia.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Monoclonal

Clone

N191/7

Isotype

IgG2b

Applications

ICC

Host Species

Mouse

Gene Name

FXN FRDA X25

Molecular Weight

25 kDa

Immunogen

Fusion protein amino acids 81-210 (C-terminus) of human Frataxin (accession number Q16595) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2939768

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

CyaY; Frataxin; FARR; EC 1.16.3.1; FRDA; Friedreich ataxia protein; X25; Fxn [Cleaved into: Frataxin intermediate form; i-FXN; mitochondrial; Frataxin(56-210; m56-FXN; Frataxin(78-210; d-FXN; m78-FXN; Frataxin mature form; Frataxin(81-210; m81-FXN

UniProt ID

Q16595
UniProt Summary: [Frataxin mature form]: Functions as an activator of persulfide transfer to the scaffoding protein ISCU as component of the core iron-sulfur cluster (ISC) assembly complex and participates to the [2Fe-2S] cluster assembly. Accelerates sulfur transfer from NFS1 persulfide intermediate to ISCU and to small thiols such as L-cysteine and glutathione leading to persulfuration of these thiols and ultimately sulfide release. Binds ferrous ion and is released from FXN upon the addition of both L-cysteine and reduced FDX2 during [2Fe-2S] cluster assembly. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. May function as an iron chaperone protein that protects the aconitase [4Fe-4S]2+ cluster from disassembly and promotes enzyme reactivation. May play a role as a high affinity iron binding partner for FECH that is capable of both delivering iron to ferrochelatase and mediating the terminal step in mitochondrial heme biosynthesis.

NCBI Gene ID

2395

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Shipped on ice packs

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Anti-Frataxin Antibody FL490 Conjugate (N191/7)

SKU: 75-207-FL490

Product Details

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