Our mouse Cav1.2 Ca2+ channel FL594 conjugate monoclonal antibody from NeuroMab is produced in-house from clone L57/46. It detects guinea pig, human, mouse, rabbit, and rat Cav1.2 Ca2+ channel and is purified by Protein A chromatography. Works in IHC, ICC.
Voltage-dependent L-type calcium channel subunit alpha-1C or Cav1.2 calcium channel (other names include as CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1 ) is a calcium channel encoded by the gene CACNA1C. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.2 is expressed in many tissues including smooth muscle, liver, kidney brain and heart. In brain, it can be detected in the hippocampus and brain cortex in the post-synaptic density and in neuronal cell bodies. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Mutations in the Cav1.2 gene have been associated with Timothy syndrome, Brugada syndrome 3 and Long QT syndrome 8.
Purified by Protein A chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Monoclonal
L57/46
IgG2b
ICC, IHC
Mouse
CACNA1C CACH2 CACN2 CACNL1A1 CCHL1A1
240 kDa (varies with cell background due to glycosylation)
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2 (accession number P15381) produced recombinantly in E. Coli
Rabbit
Guinea, Guinea Pig, Human, Mouse, Rat
AB_2939234
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL594 Ex: 594 nm, Em: 615 nm
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
P15381
UniProt Summary: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Mediates influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. Plays an important role in excitation-contraction coupling in the heart. Required for normal heart development and normal regulation of heart rhythm. Required for normal contraction of smooth muscle cells in blood vessels and in the intestine. Essential for normal blood pressure regulation via its role in the contraction of arterial smooth muscle cells. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group (Probable).
