Anti-Caspase-3 (N-terminal region) Antibody (M377)

Name: Anti-Caspase-3 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM3771
Price: 292.0 USD
Availability: InStock

Our Caspase-3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Caspase-3 (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Inhibition of protein biosynthesis as shown by puromycin Western blot analysis and induction of apoptosis in LNCaP cells as demonstrated by PARP and Caspase-3 (Cat. CM3771) cleavage after combination treatment. Image from publication CC-BY-4.0. PMID: 37484018

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: CM3771

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Caspase-3 (N-terminal region)

Scientific Background

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M377

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

CASP3

Molecular Weight

Immunogen

Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CPP32; Caspase,-3; SCA-1; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3; apoptosis-related cysteine peptidase; apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama; Apoptain; Yama; CASP-2; NEDD2; ICH-1; p18; p13; p12

UniProt ID

P42574
UniProt Summary: Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). Cleaves and inactivates interleukin-18 (IL18). Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves DSG2 in response to apoptosis resulting in a loss of full length DSG2 at desmosome cell junctions and subsequent loss of cell-cell adhesion. Also cleaves JUP in response to apoptosis. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC.

NCBI Gene ID

836

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Anti-Caspase-3 (N-terminal region) Antibody (M377)

SKU: CM3771

Product Details

Bulk Order Anti-Caspase-3 (N-terminal region) Antibody

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