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Antibodies Incorporated SynaptoGreenTM C18 (equivalent to FM®3-25) Nerve Terminal Dye

From $509

Antibodies Incorporated SynaptoGreenTM C2 (equivalent to FM®2-10) Nerve Terminal Dye

From $336

Antibodies Incorporated SynaptoGreenTM C3 Nerve Terminal Dye

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Antibodies Incorporated SynaptoGreenTM C4 (equivalent to FM®1-43) Nerve Terminal Dye

From $438

Antibodies Incorporated SynaptoGreenTM C4 Nerve Terminal Staining Kit I

$662

Antibodies Incorporated SynaptoGreenTM C4 Nerve Terminal Staining Kit III

$560

Antibodies Incorporated SynaptoGreenTM C5 Nerve Terminal Dye

From $336

Antibodies Incorporated SynaptoRedTM C1 Nerve Terminal Dye

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Antibodies Incorporated SynaptoRedTM C2 (equivalent to FM®4-64) Nerve Terminal Dye

From $438

Antibodies Incorporated SynaptoRedTM C2 Nerve Terminal Staining Kit V

$662

Antibodies Incorporated SynaptoRedTM C2M (equivalent to FM®5-95) Nerve Terminal Dye

From $438

PhosphoSolutions Talin Phospho-Regulation Antibody Kit

$470

ImmunoChemistry Technologies Tetramethylbenizidine IHC Peroxidase

From $89.95

Antibodies Incorporated Tetrodotoxin with Citrate

$285

Antibodies Incorporated Tetrodotoxin, Citrate Free

$285

Antibodies Incorporated Thioflavin T Amyloid Stain, high purity grade

$121

ImmunoChemistry Technologies TMB 1-Component HRP Membrane Substrate (SUBM)

$67.45

ImmunoChemistry Technologies TMB 1-Component HRP Microwell Substrate (SUBT)

From $47.10

ImmunoChemistry Technologies TMB Slow Kinetic 1-Component HRP Microwell Substrate (SUBK)

$59.45

ImmunoChemistry Technologies TMB Super Sensitive 1-Component HRP Microwell Substrate (SUBS)

From $49.25

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine to induce apoptosis, then stained with TMRE, and photographed using a photomicroscope using a green excitation filter at 510-560 nm and a 570-620 nm emission filter (left). Healthy cells, containing mitochondria with polarized inner membranes fluoresce bright orange (A) compared with apoptotic cells with depolarized mitochondria(B). The corresponding DIC images are shown (right) (Dr. Brian Lee, ICT).

Figure 2. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRE - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$305.60

Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (Cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$305.60

ImmunoChemistry Technologies Tris Buffer Saline with Tween, 10X (TBST)

From $61.30

ImmunoChemistry Technologies Tris-HCL Buffer, 10X

From $61.30

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