Anti-Phosphoserine/threonine Antibody

Name: Anti-Phosphoserine/threonine Antibody
Brand: PhosphoSolutions
SKU: PP2551
Price: 348.0 USD
Availability: InStock

Our Anti-Phosphoserine/threonine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is antigen affinity purified. It is great for use in WB, ICC, IP.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.

Western blot showing N2B-Titin total phosphorylation (Cat. PP2551, 1:500) in male and female mRen2 and WT rats. Image from publication CC-BY-4.0. PMID: 37363100

Western blot image of plant extracts with intact FonKin4 and truncated S_TKc domain-containing fragment FonKin4-ST show Ser/Thr protein kinase activity detected by anti-phospho Ser/Thr antibody (Cat. PP2551). FonKin4 is a Ser/Thr protein kinase and its S_TKc domain is sufficient for its pathogenicity function in Fon. Image from publication CC-BY-4.0. PMID: 38690366.

Purified His-tagged NFR1, NFR1Y429F, or NFR1T481A CDs were incubated with ATP for 30 min. The proteins were detected by immunoblotting with anti-His antibody. Phosphorylated products were detected using anti-pSer/Thr (Cat. PP2551) or anti-pTyr antibodies. Un-phosphorylated NFR1-CD band is indicated by the red triangle. Image from publication CC-BY-4.0. PMID: 38670968

Western blot of Citrus sinensis "sweet orange" calcineurin B-like protein kinase 23 (CsCIPK23) phosphorylates C. sinensis tonoplast-localized sugar transporter 2 (CsTST2) at the Ser277, Ser337, Ser354 residues. Phosphorylated CsTST2 protein was detected by anti-pSer/pThr antibody (Cat. PP2551). Image from publication, CC-BY-4.0. PMID: 39611527.

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IP, WB

Host Species

Rabbit

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Data Sheet

SKU: PP2551

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Anti-Phosphoserine/threonine

Scientific Background

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IP, WB

Host Species

Rabbit

Gene Name

N/A

Molecular Weight

N/A

Immunogen

Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

pSer/Thr

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Phosphoser/thr mAb

UniProt ID

N/A

Shipping

Blue Ice

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Anti-Phosphoserine/threonine Antibody

SKU: PP2551

Product Details

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