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Adult rat brain immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged SynDIG3 or untagged Kv2.1 plasmid and probed with N160/21 (left) and K89/41 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Tafazzin or untagged Kv2.1 plasmid and probed with N173B/13 (left) and 12CA5 (right) TC supe.
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody (TM4081), then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).

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