Our Anti-NMDA NR2B Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2B Subunit and is neat pooled serum. It is great for use in WB, ICC.
Immunofluorescence of healthy iPSC-derived neurons labeled with Anti-NMDA Receptor, NR2B subunit (Cat. 1498-NR2B, green, 1:100) and Anti-TuJ1 (red). The blue is Hoechst staining of nuclear DNA. Image kindly provided by Dr. Allison Ebert, Medical College of Wisconsin.
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Neat Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
GRIN2B
180 kDa
Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor.
Rat
Human, Mouse, Rat
AB_2492177
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Unpurified pooled neat serum.
Neat serum
WB: 1:1000
WB Brain: 1:1000
ICC: 1:100
Unconjugated
Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
