Anti-KCC2 Antibody (N1/12)

Name: Anti-KCC2 Antibody
Brand: NeuroMab
SKU: 75-013
Price: 394.0 USD
Availability: InStock

Our mouse KCC2 monoclonal antibody from NeuroMab is produced in-house from clone N1/12. It detects finch, human, mouse, and rat KCC2 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat cerebellum immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

HEK-293 cells were transiently transfected with rnKCC2bWT or rnKCC2 variants with mutations in the chloride-binding site 1. Immunocytochemistry was used to monitor the transfection rate of the rnKCC2 variants (Cat. 75-013, 1:1000; green) and cell staining by DAPI (blue). Image from publication CC-BY-4.0. PMID:37625593

Immunocytochemistry was used to monitor the transfection rate of KCC2 (Cat. 75-013, 1:1000; green) and cell staining by DAPI (blue) in HEK293 cells transiently transfected with KCC2aWT or KCC2a Thr934Ala/Ser937Asp. Image from publication CC-BY-4.0. PMID:38066086

Adult rat cerebellum immunohistochemistry.

Click on image to zoom

Species Reactivity

Finch, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-013

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

KCC2

Scientific Background

KCC2 or Potassium-chloride transporter member 5 is a member of the chloride potassium symporter family. KCC2 is a neuron-specific membrane protein encoded by the SLC12A5 gene in humans. The protein colocalizes with GABAA receptors and has for function to mediates electroneutral potassium-chloride cotransport in mature neurons to mainatin the Cl- homeostasis. KCC2 is widely expressed throughout the central nervous system, especially the hippocampus, hypothalamus, brainstem, and motoneurons of the ventral spinal cord. KCC2 is a neuronal marker that is often used to measure post-synaptic inhibition through GABAA receptors.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N1/12

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc12a5 Kcc2 Kiaa1176

Molecular Weight

140 kDa

Immunogen

Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2 (accession number Q63633) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Finch, Human, Mouse, Rat

Antibody Registry ID

AB_10672851

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)

UniProt ID

Q63633

Shipping

Shipped on ice packs

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Anti-KCC2 Antibody (N1/12)

SKU: 75-013

Product Details

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