Products: Primary Antibodies

Anti-WAVE1/SCAR Antibody (K91/36)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-048	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-048	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K91/36 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

Wiskott-Aldrich syndrome protein family member 1, Verprolin Homology Domain-Containing Protein 1, Protein WAVE-1 or SCAR1 is encoded by the gene WASF1 and is a member of the Wiskott-Aldrich syndrome protein (WASP)-family. WAVE1/SCAR is an effector protein which transmits of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. WAVE1/SCAR acts as part of the WAVE complex which is involved in the regulation of actin filament reorganization and membrane ruffling. WAVE1/SCAR is expressed in many tissues including brain, ovary, colon, kidney, pancreas and thymus. Diseases associated with this gene include Wiskott-Aldrich Syndrome and Alacrima, Achalasia, And Mental Retardation Syndrome.

Immunogen:

Synthetic peptide amino acids 477-498 of human Wave1/Wasp family 1(CQVIPASEPKRHPSTLPVISDAR; accession number Q92558); Rat: 95% identity (21/22); Mouse=95% identity (21/22)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

WAVE1/SCAR

Gene Name(s):

WASF1 KIAA0269 SCAR1 WAVE1

Antibody Registry ID:

Purified: AB_2216976
TC Supernatant: AB_10671317

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.