Products: Primary Antibodies

Anti-WAVE1/SCAR (K91/36)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-048	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-048	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K91/36 (left) or N52A/42 (right) TC supe.$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Synthetic peptide amino acids 477-498 of human Wave1/Wasp family 1(CQVIPASEPKRHPSTLPVISDAR; accession number Q92558)
Rat: 95% identity (21/22)
Mouse=95% identity (21/22)

Cross Reactivity:

None Reported

Protein Name(s):

Wiskott-Aldrich syndrome protein family member 1 (WASP family protein member 1) (Protein WAVE-1) (Verprolin homology domain-containing protein 1)

Gene Name(s):

WASF1 KIAA0269 SCAR1 WAVE1

Antibody Registry ID:

Purified: AB_2216976
TC Supernatant: AB_10671317

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Hsiao K, Harony-Nicolas H, Buxbaum JD, Bozdagi-Gunal O, Benson DL.. (2016), 'Cyfip1 Regulates Presynaptic Activity during Development. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0511-15.2016.
Chazeau A, Mehidi A, Nair D, Gautier JJ, Leduc C, Chamma I, Kage F, Kechkar A, Thoumine O, Rottner K, Choquet D, Gautreau A, Sibarita JB, Giannone G.. (2014), 'Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion. .' The EMBO Journal. 10.15252/embj.201488837.
Chia PH, Chen B, Li P, Rosen MK, Shen K.. (), 'Local F-actin network links synapse formation and axon branching. .' Cell. 10.1016/j.cell.2013.12.009.
Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK.. (), 'The WAVE regulatory complex links diverse receptors to the actin cytoskeleton. .' Cell. 10.1016/j.cell.2013.11.048.
Han K, Holder JL Jr, Schaaf CP, Lu H, Chen H, Kang H, Tang J, Wu Z, Hao S, Cheung SW, Yu P, Sun H, Breman AM, Patel A, Lu HC, Zoghbi HY.. (2013), 'SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. .' Nature. 10.1038/nature12630.
Khodosevich K, Seeburg PH, Monyer H.. (2009), 'Major signaling pathways in migrating neuroblasts. .' Frontiers in Molecular Neuroscience. 10.3389/neuro.02.007.2009.
Cheng A, Arumugam TV, Liu D, Khatri RG, Mustafa K, Kwak S, Ling HP, Gonzales C, Xin O, Jo DG, Guo Z, Mark RJ, Mattson MP.. (2007), 'Pancortin-2 interacts with WAVE1 and Bcl-xL in a mitochondria-associated protein complex that mediates ischemic neuronal death. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5154-06.2007.