Products: Primary Antibodies

Anti-Slc18a2/VMAT2 Antibody (N449/73)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-436	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-436	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Target Description:

Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005).

Immunogen:

Fusion protein amino acids 42-132 (first lumenal domain) of mouse Slc18a2 (also known as Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, VAT2, Svmt, Svat and Vmat2, accession number Q66L40)
Rat: 82% identity (75/92 amino acids identical)
Human: 73% identity (67/92 amino acids identical)
30% identity with Slc18a1/VMAT1

Cross Reactivity:

Does not cross-react with Slc18a1/VMAT1 or Slc18a3/VAChT

Protein Name(s):

Slc18a2 protein

Gene Name(s):

Slc18a2

Antibody Registry ID:

Purified: AB_2686911
TC Supernatant: AB_2532092

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.