Products: Primary Antibodies

Anti-Pan-Neurofascin (extracellular) (A12/18)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-172
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$219.00
Catalog #
73-172
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$189.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Immunogen:

Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by: - binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186)
Mouse: 98% identity (1074/1086 amino acids identical)
Human: 79% identity (955/1195 amino acids identical)
This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Cross Reactivity:

None Reported

Protein Name(s):

Neurofascin NF-155 NF-186

Gene Name(s):

Nfasc

Antibody Registry ID:

Purified: AB_2282826
TC Supernatant: AB_10671311


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

155, 186 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.