Products: Primary Antibodies

Anti-Notch1 (N253/32)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-295
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-295
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Immunogen:

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (also known as Neurogenic locus notch homolog protein 1, hN1, Translocationassociated notch protein, TAN-1, Motch A, mT14, p300 and Motch, accession number Q01705) Rat: 93% identity (185/197 amino acids identical) Human: 84% identity (166/197 amino acids identical) ~40% identity with Notch2, Notch3 and Notch4

Cross Reactivity:

Does not cross-react with Notch2 or Notch3

Protein Name(s):

Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT); Notch 1 intracellular domain (NICD)]

Gene Name(s):

Notch1 Motch

Antibody Registry ID:

Purified: AB_2315898
TC Supernatant: AB_2315897


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

>270 kDa, ~120 kDa and smaller fragments (due to proteolysis)


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.