Anti-Notch1 Antibody FL490 Conjugate (N253/32)

Name: Anti-Notch1 Antibody FL490 Conjugate
Brand: NeuroMab
SKU: 75-295-FL490
Price: 486.0 USD
Availability: InStock

Our mouse Notch1 FL490 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N253/32. It detects mouse and rat Notch1 and is purified by Protein A chromatography. Works in ICC.

Anti-Notch1 Antibody FL490 Conjugate

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Species Reactivity

Mouse, Rat

Applications

ICC

Host Species

Mouse

Conjugates and Formulations

Unconjugated

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-295-FL490

Volume: 200 µL

Variant

Price:

$486.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Notch1

Scientific Background

Notch Receptor 1 is encoded by the gene NOTCH1. NOTCH1 is a member of the NOTCH family. NOTCH1 is involved in processes related to cell fate specification, differentiation, proliferation, and survival. NOTCH1 is highly expressed in the brain, lungs, and thymus. Diseases associated with NOTCH1 include Aortic Valve Disease 1 and Adams-Oliver Syndrome 5.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Monoclonal

Clone

N253/32

Isotype

IgG1

Applications

ICC

Host Species

Mouse

Gene Name

Notch1 Motch

Molecular Weight

>270 kDa, ~120 kDa and smaller fragments (due to proteolysis)

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (accession number Q01705) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2940036

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL490 Ex: 491 nm, Em: 515 nm

Specificity

Does not cross-react with Notch2 or Notch3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

9930111A19Rik; Neurogenic locus notch homolog protein 1; Tan1; Notch 1; lin-12; Motch A; mT14; p300 [Cleaved into: Notch 1 extracellular truncation; Notch gene homolog 1; NEXT; Notch 1 intracellular domain; major type A protein; NICD; p300; transmembrane receptor Notch1

UniProt ID

Q01705
UniProt Summary: Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

NCBI Gene ID

18128

Shipping

Shipped on ice packs

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Anti-Notch1 Antibody FL490 Conjugate (N253/32)

SKU: 75-295-FL490

Product Details

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