Products: Primary Antibodies

Anti-Neuroligin-1 (N97A/31)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-160	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-160	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 718-843 (intracellular C-terminus) of rat Neuroligin-1 (accession number NP_446320)
Mouse: 99% identity (125/126 amino acids identical)
Human: 99% identity (125/126 amino acids identical)
~60% identity with Neuroligin-3
~40% identity with Neuroligin-2

Cross Reactivity:

Does not cross-react with Neuroligin-2, -3, -4 or -4*

Protein Name(s):

Neuroligin-1 (Neuroligin I)

Gene Name(s):

Nlgn1

Antibody Registry ID:

Purified: AB_2235964
TC Supernatant: AB_10671307

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

120 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Burch A, Tao-Cheng JH, Dosemeci A.. (2017), 'A novel synaptic junction preparation for the identification and characterization of cleft proteins. .' PLoS One. 10.1371/journal.pone.0174895.
Um JW, Choii G, Park D, Kim D, Jeon S, Kang H, Mori T, Papadopoulos T, Yoo T, Lee Y, Kim E, Tabuchi K, Ko J.. (2016), 'IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation. .' The Journal of Biological Chemistry. 10.1074/jbc.M115.712893.
Hoy JL, Haeger PA, Constable JR, Arias RJ, McCallum R, Kyweriga M, Davis L, Schnell E, Wehr M, Castillo PE, Washbourne P.. (2013), 'Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4660-12.2013.
Yamagata M, Duan X, Sanes JR.. (2018), 'Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation. .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2018.00142.
Zou D,,, McSweeney C, Sebastian A, Reynolds DJ, Dong F, Zhou Y, Deng D,, Wang Y1, Liu L1,1, Zhu J1, Zou J1, Shi Y1, Albert I1, Mao Y1,1.. (2015), 'A critical role of RBM8a in proliferation and differentiation of embryonic neural progenitors. .' Neural Development. 10.1186/s13064-015-0045-7.
de Andrade GB, Kunzelman L, Merrill MM, Fuerst PG.. (2014), 'Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. .' Molecular Vision. .
Li Q, Zheng S, Han A, Lin CH, Stoilov P, Fu XD, Black DL.. (2014), 'The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. .' Elife. 10.7554/eLife.01201.
Lugo JN, Smith GD, Arbuckle EP, White J, Holley AJ, Floruta CM, Ahmed N, Gomez MC, Okonkwo O.. (2014), 'Deletion of PTEN produces autism-like behavioral deficits and alterations in synaptic proteins. .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2014.00027.
Li Q, Zheng S, Han A, Lin CH, Stoilov P, Fu XD, Black DL.. (2014), 'The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. .' Elife. 10.7554/eLife.01201.
Yamagata M, Sanes JR.. (2012), 'Transgenic strategy for identifying synaptic connections in mice by fluorescence complementation (GRASP). .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2012.00018.