Products: Primary Antibodies

Anti-Neuroligin-1 Antibody (N97A/31)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-160	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-160	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Neuroligin 1 is a member of a large family of alternatively sliced proteins located at the post synaptic surface in the central nervous system, and stains as a post synaptic marker. Neuroligin 1 is involved in cell-cell-interactions by binding to neurexin family members and other postsynaptic proteins to localize receptors and channels in the postsynaptic density. Suggested roles for Neuroligin 1 include synapse function and synaptic signal transmission and maintaining synapse integrity. Mutations in Neuroligans have been implicated in autism and other cognitive disorders.

Immunogen:

Fusion protein amino acids 718-843 (intracellular C-terminus) of rat Neuroligin-1 (accession number NP_446320); Mouse: 99% identity (125/126 amino acids identical); Human: 99% identity (125/126 amino acids identical); ~60% identity with Neuroligin-3; ~40% identity with Neuroligin-2

Cross Reactivity:

Does not cross-react with Neuroligin-2, -3, -4 or -4*

Protein Name(s):

Neuroligin-1

Gene Name(s):

Nlgn1

Antibody Registry ID:

Purified: AB_2235964
TC Supernatant: AB_10671307

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

120 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.