Products: Primary Antibodies

Anti-Navbeta3 Na+ channel Antibody (N396/29)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-391	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-391	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Target Description:

Sodium channel subunit beta-3 or Navbeta3 Na+ channel is encoded by SCN3B. Voltage gated sodium channels are made up of an alpha (pore forming) subunit and 1 or 2 beta subunits. Navß3 is a transmembrane protein and acts as a regulatory subunit in excitable membranes and modulates the kinetics of channel. Nabß3 is expressed in many tissues, including the central and peripheral nervous system, heart and pancreatic endocrine cells. Diseases associated with SCN3A include forms of Epilepsy and Encephalopathy.

Immunogen:

Fusion protein amino acids 1-215 (full-length) of rat Navbeta3 (also known as Sodium channel subunit beta-3, Scn3b and KIAA1158, accession number Q9JK00) Mouse: 99% identity (214/215 amino acids identical)
Human: 98% identity (211/215 amino acids identical)
50% identity with Navbeta1, Navbeta2 or Navbeta4

Cross Reactivity:

Does not cross-react with Navbeta1, Navbeta2 or Navbeta4

Protein Name(s):

Sodium channel subunit beta-3

Gene Name(s):

Scn3b

Antibody Registry ID:

Purified: AB_2491090
TC Supernatant: AB_2336910

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

40 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.