Products: Primary Antibodies

Anti-NALCN (N185/7)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-220	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-220	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN (also known as Sodium leak channel non-selective protein, Four domain-type voltage-gated ion channel alpha-1 subunit, Voltage-gated channel-like protein 1, Vgcnl1, Nca and Cankon, accession number Q6Q760)
Mouse: 98% identity (79/80 amino acids identical)
Human: 95% identity (76/80 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Sodium leak channel non-selective protein (Four domain-type voltage-gated ion channel alpha-1 subunit) (Rb21-channel) (Voltage gated channel-like protein 1)

Gene Name(s):

Nalcn Nca Rb21 Vgcnl1

Antibody Registry ID:

Purified: AB_2149984
TC Supernatant: AB_10672975

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.