Human Mouse Rat
ICC IHC WB
Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse Lgi1 (also known as Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT and UNQ775/PRO1569, accession number Q9JIA1)
Rat: 100% identity (77/77 amino acids identical)
Human: 98% identity (76/77 amino acids identical)
~50% identity with Lgi2, Lgi3 and Lgi4
Does not cross-react with Lgi2, Lgi3 or Lgi4 (based on KO validation results)
Leucine-rich glioma-inactivated protein 1
TC Supernatant: AB_2315875
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.