Products: Primary Antibodies

Anti-Lgi1 Antibody (N283/7)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-307
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-307
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Leucine Rich Glioma Inactivated 1 is encoded by the gene LGI1. LGI1 is a member of the secreted leucine-rich repeat (LRR) superfamily. LGI1 may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. LGI1 is expressed in the brain. Diseases associated with LGI1 include Epilepsy, Familial Temporal Lobe, 1 and Autosomal Dominant Epilepsy with Auditory Features.

Immunogen:

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse Lgi1 (also known as Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT and UNQ775/PRO1569, accession number Q9JIA1)
Rat: 100% identity (77/77 amino acids identical)
Human: 98% identity (76/77 amino acids identical)
~50% identity with Lgi2, Lgi3 and Lgi4

Cross Reactivity:

Does not cross-react with Lgi2, Lgi3 or Lgi4 (based on KO validation results)

Protein Name(s):

Leucine-rich glioma-inactivated protein 1

Gene Name(s):

Lgi1

Antibody Registry ID:

Purified: AB_2315876
TC Supernatant: AB_2315875


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.