Products: Primary Antibodies

Anti-Kv4.2 potassium channel Antibody (L28/4)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-361	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-361	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebellum [HBM(Cb)] or cerebral cortex [HBM(Cx)] and probed with L28/4 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Potassium voltage-gated channel subfamily D member 2 or Kv4.2 potassium channel subunit is encoded by the gene KCND2. Kv4.2 is an alpha subunit of the voltage-gated potassium channel (Shal) family and mediates transmembrane potassium transport in excitable membranes. Kv4.2 is an integral membrane protein that combines to form tetramers. Kv4.2 is detected in the heart and brain. In brain it is found at the cell junction, synapse, postsynaptic cell membrane. Diseases associated with this gene include Cycloplegia and early myoclonic encephalopathy.

Immunogen:

Fusion protein amino acids 471-630 (cytoplasmic C-terminus) of rat Kv4.2 (Potassium voltage-gated channel subfamily D member 2, Voltage-gated potassium channel subunit Kv4.2, Kcnd2, Kiaa1044, MNCb-7013, RK5 and Shal1, accession number Q63881) Mouse: 99% identity (159/160 amino acids identical)
Human: 98% identity (157/160 amino acids identical)
<50% identity with Kv4.3

Cross Reactivity:

Does not cross-react with Kv4.3 (based on KO validation results)

Protein Name(s):

Potassium voltage-gated channel subfamily D member 2 (RK5) (Shal1) (Voltage-gated potassium channel subunit Kv4.2)

Gene Name(s):

Kcnd2

Antibody Registry ID:

Purified: AB_2315874
TC Supernatant: AB_2315873

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.