Products: Primary Antibodies

Anti-Kv1.3 K+ channel (L23/27)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-009	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-009	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Synthetic peptide amino acids 485-506 (GGMNHSAFPQTPFKTGNSTATC, cytoplasmic C-terminus) of rat Kv1.3 (also known as Potassium voltage-gated channel subfamily A member 3, Voltage-gated potassium channel subunit Kv1.3, Kv3, KIII, GK5, K3 and Kcna3, accession number P15384)

Human: 100% identity (22/22 amino acids identical)
Mouse: 100% identity (22/22 amino acids identical)

Protein Name(s):

Potassium voltage-gated channel subfamily A member 3 (RCK3) (RGK5) (Voltage-gated potassium channel subunit Kv1.3) (Voltage-gated potassium channel subunit Kv3)

Gene Name(s):

Kcna3

Antibody Registry ID:

Purified: AB_2133637
TC Supernatant: AB_10673575

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Kang JA, Park SH, Jeong SP, Han MH, Lee CR, Lee KM, Kim N, Song MR, Choi M, Ye M, Jung G, Lee WW, Eom SH, Park CS, Park SG.. (2016), 'Epigenetic regulation of Kcna3-encoding Kv1.3 potassium channel by cereblon contributes to regulation of CD4+ T-cell activation..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1502166113.
Xu L, Schaefer ML, Linville RM, Aggarwal A, Mbuguiro W, Wester BA, Koliatsos VE.. (2016), 'Neuroinflammation in primary blast neurotrauma: Time course and prevention by torso shielding..' Experimental Neurology. 10.1016/j.expneurol.2016.01.010.
Jiménez-Pérez L, Cidad P, Álvarez-Miguel I, Santos-Hipólito A, Torres-Merino R, Alonso E, de la Fuente MÁ, López-López JR, Pérez-García MT.. (2016), 'Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation..' The Journal of Biological Chemistry. 10.1074/jbc.M115.678995.
Martínez-Mármol R,, Comes N, Styrczewska K, Pérez-Verdaguer M, Vicente R, Pujadas L, Soriano E,,, Sorkin A, Felipe A,8. (2016), 'Unconventional EGF-induced ERK1/2-mediated Kv1.3 endocytosis. .' Cell Mol Life Sciences. 10.1007/s00018-015-2082-0.
Cidad P, Novensà L, Garabito M, Batlle M, Dantas AP, Heras M, López-López JR, Pérez-García MT, Roqué M.. (2014), 'K+ channels expression in hypertension after arterial injury, and effect of selective Kv1.3 blockade with PAP-1 on intimal hyperplasia formation..' Cardiovascular Drugs and Therapy. 10.1007/s10557-014-6554-5.
Kundu-Raychaudhuri S, Chen YJ, Wulff H, Raychaudhuri SP.. (2014), 'Kv1.3 in psoriatic disease: PAP-1, a small molecule inhibitor of Kv1.3 is effective in the SCID mouse psoriasis--xenograft model..' Journal of Autoimmunity. 10.1016/j.jaut.2014.07.003.
Dufour MA, Woodhouse A, Goaillard JM.. (2014), 'Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development..' Journal of Neuroscience Research. 10.1002/jnr.23382.
Peng Y, Lu K, Li Z, Zhao Y, Wang Y, Hu B, Xu P, Shi X, Zhou B, Pennington M, Chandy KG, Tang Y.. (2014), 'Blockade of Kv1.3 channels ameliorates radiation-induced brain injury..' Neuro-Oncology. 10.1093/neuonc/not221.
Hamilton DL, Beall C, Jeromson S, Chevtzoff C, Cuthbertson DJ, Ashford ML.. (2014), 'Kv1.3 inhibitors have differential effects on glucose uptake and AMPK activity in skeletal muscle cell lines and mouse ex vivo skeletal muscle..' The Journal of Physiology Sci.. 10.1007/s12576-013-0285-4.
Duque A, Gazula VR, Kaczmarek LK.. (2014), 'Expression of Kv1.3 potassium channels regulates density of cortical interneurons..' Developmental Neurobiology. 10.1002/dneu.22105.
Ovsepian SV, Steuber V, Le Berre M, O'Hara L, O'Leary VB, Dolly JO.. (2014), 'A defined heteromeric KV1 channel stabilizes the intrinsic pacemaking and regulates the output of deep cerebellar nuclear neurons to thalamic targets..' The Journal of Physiology. 10.1113/jphysiol.2012.249706.
Nguyen HM, Miyazaki H, Hoshi N, Smith BJ, Nukina N, Goldin AL, Chandy KG.. (2012), 'Modulation of voltage-gated K+ channels by the sodium channel β1 subunit..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1209142109.
Yamada J, Jinno S.. (2013), 'Novel objective classification of reactive microglia following hypoglossal axotomy using hierarchical cluster analysis..' Journal of Comparative Neurology. 10.1002/cne.23228.
Zhu J, Yan J, Thornhill WB.. (2012), 'N-glycosylation promotes the cell surface expression of Kv1.3 potassium channels..' The FEBS Journal. 10.1111/j.1742-4658.2012.08642.x.
He S, Shao LR, Rittase WB, Bausch SB.. (2012), 'Increased Kv1 channel expression may contribute to decreased sIPSC frequency following chronic inhibition of NR2B-containing NMDAR..' Neuropsychopharmacology. 10.1038/npp.2011.320.
Roepke TK, Kanda VA, Purtell K, King EC, Lerner DJ, Abbott GW.. (2011), 'KCNE2 forms potassium channels with KCNA3 and KCNQ1 in the choroid plexus epithelium..' The FASEB Journal. 10.1096/fj.11-187609.
Straub SV, Perez SM, Tan B, Coughlan KA, Trebino CE, Cosgrove P, Buxton JM, Kreeger JM, Jackson VM.. (2011), 'Pharmacological inhibition of Kv1.3 fails to modulate insulin sensitivity in diabetic mice or human insulin-sensitive tissues..' Am The Journal of Physiology Endocrinol Metab.. 10.1152/ajpendo.00076.2011.
Ramirez-Navarro A, Glazebrook PA, Kane-Sutton M, Padro C, Kline DD, Kunze DL.. (2011), 'Kv1.3 channels regulate synaptic transmission in the nucleus of solitary tract..' Journal of Neurophysiology. 10.1152/jn.00494.2010.
Fulton S, Thibault D, Mendez JA, Lahaie N, Tirotta E, Borrelli E, Bouvier M, Tempel BL, Trudeau LE.. (2011), 'Contribution of Kv1.2 voltage-gated potassium channel to D2 autoreceptor regulation of axonal dopamine overflow..' The Journal of Biological Chemistry. 10.1074/jbc.M110.153262.
Hyodo T, Oda T, Kikuchi Y, Higashi K, Kushiyama T, Yamamoto K, Yamada M, Suzuki S, Hokari R, Kinoshita M, Seki S, Fujinaka H, Yamamoto T, Miura S, Kumagai H.. (2010), 'Voltage-gated potassium channel Kv1.3 blocker as a potential treatment for rat anti-glomerular basement membrane glomerulonephritis..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00374.2010.
Gazula VR, Strumbos JG, Mei X, Chen H, Rahner C, Kaczmarek LK.. (2010), 'Localization of Kv1.3 channels in presynaptic terminals of brainstem auditory neurons..' Journal of Comparative Neurology. 10.1002/cne.22393.
Wang T, Lee MH, Johnson T, Allie R, Hu L, Calabresi PA, Nath A.. (2010), 'Activated T-cells inhibit neurogenesis by releasing granzyme B: rescue by Kv1.3 blockers..' Journal of Neuroscience. 10.1523/JNEUROSCI.0311-10.2010.
Cidad P, Moreno-Domínguez A, Novensá L, Roqué M, Barquín L, Heras M, Pérez-García MT, López-López JR.. (2010), 'Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells..' Arterioscler Thromb Vasc Biol.. 10.1161/ATVBAHA.110.205187.
Miller MN, Okaty BW, Nelson SB.. (2008), 'Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits..' Journal of Neuroscience. 10.1523/JNEUROSCI.2940-08.2008.
Doczi MA, Morielli AD, Damon DH.. (2008), 'Kv1.3 channels in postganglionic sympathetic neurons: expression, function, and modulation..' Am The Journal of Physiology Regul Integr Comp Physiol.. 10.1152/ajpregu.00077.2008.