Products: Primary Antibodies

Anti-Kv1.3 K+ channel Antibody (L23/27)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-009
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-009
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

Kv1.3 K+ channel (also known KCNA3) is a member of the potassium channel, voltage-gated, shaker-related subfamily. This protein, encoded by the KCNA3 gene in human, is an efficient channel blocker. Kv1.3 K+ channel includes a shaker-type repeat in the fourth segment of a six membrane-spanning domains. Kv1.3 K+ channel plays a role in the repolarization of nerve cells after an action potential. It is also widely expressed on T and B lymphocytes'membranes (including inner mitochondrial membrane) and has an important function in T-cell activation, especially in the regulation of chronically activated effector memory T-cell immune responses (Chiang et al., 2017). It has been demonstrated that effector memory T-cells highly expressing Kv1.3 K+ channel are predominantely present in patient with Musltiple Sclerosis lesion and children with type-1 diabetes mellitus (Beeton et al., 2006).

Immunogen:

Synthetic peptide amino acids 485-506 (GGMNHSAFPQTPFKTGNSTATC, cytoplasmic C-terminus) of rat Kv1.3 (also known as Potassium voltage-gated channel subfamily A member 3, Voltage-gated potassium channel subunit Kv1.3, Kv3, KIII, GK5, K3 and Kcna3, accession number P15384)
Human: 100% identity (22/22 amino acids identical)
Mouse: 100% identity (22/22 amino acids identical)

Protein Name(s):

Kv1.3 K+ channel

Gene Name(s):

Kcna3

Antibody Registry ID:

Purified: AB_2133637
TC Supernatant: AB_10673575


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

70 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.