Products: Primary Antibodies

Anti-Kv1.2 K+ Channel Antibody (K14/16)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

★ Trial Size Available ★
Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-008
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$325.00
Brand
NeuroMab Logo
Catalog #
75-008-020
Option
Purified Trial-Size
Volume
20 µL
Concentration
1 mg/mL
Price
$109.00
Brand
NeuroMab Logo
Catalog #
73-008
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$325.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat Xenopus Zebrafish

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

Kv1.2 K+ channel (Potassium voltage-gated channel subfamily A member 2), which is encoded by KCNA2 gene, is part of the Potassium voltage-gated channel family. Kv1.2 K+ channel switches between its open and closed conformation in response to the voltage difference across the membrane, which selectively allows the traffic of K+. Kv1.2 K+ channel determines potassium ion permeability of excitable membranes according to their electrochemical gradient. Therefore, this protein has for function to regulate neurotransmitter release, neuronal excitability, smooth muscle contraction, heart muscle contraction or insulin secretion. Kv1.2 is recognized as a tansmembrane/ multi-pass membrane protein. Anti-Kv1.2 K+ channel K14/16 is often used in IHC on human normal cerebral cortex.

Immunogen:

Fusion protein amino acids 428-499(cytoplasmic C-terminus) of rat Kv1.2 (accession number P63142), Epitope mapped to within amino acids 463-480; Human: 100% identity; Mouse: 100% identity

Cross Reactivity:

No cross-reactivity against Kv1.1, Kv1.3, Kv1.4, Kv1.5 and Kv1.6 expressed in transfected cells

Protein Name(s):

Kv1.2 K+ channel

Gene Name(s):

KCNA2

Antibody Registry ID:

Purified: AB_2296313
Purified Trial-Size: AB_2296313
TC Supernatant: AB_10674277


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

80 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.