Products: Primary Antibodies

Anti-Kir2.3 K+ channel (N25/35)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-069	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-069	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Rat

Applications:

IHC WB

Immunogen:

Fusion protein amino acids 390-445 of human Kir2.3 (accession number NP_032453)
Mouse: 96% identity (55/57 amino acids identical)
Rat: 94% identity (54/57 amino acids identical)

Cross Reactivity:

No cross-reactivity against Kir2.1 or Kir2.2

Protein Name(s):

Inward rectifier potassium channel 4 (HIRK2) (HRK1) (Hippocampal inward rectifier) (HIR) (Inward rectifier K(+) channel Kir2.3) (IRK-3) (Potassium channel, inwardly rectifying subfamily J member 4)

Gene Name(s):

KCNJ4 IRK3

Antibody Registry ID:

Purified: AB_2130742
TC Supernatant: AB_10673968

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Sivagangabalan G, Nazzari H, Bignolais O, Maguy A, Naud P, Farid T, Massé S, Gaborit N, Varro A, Nair K, Backx P, Vigmond E, Nattel S, Demolombe S, Nanthakumar K.. (2014), 'Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. .' PLoS One. 10.1371/journal.pone.0082179.
Cazorla M, Shegda M, Ramesh B, Harrison NL, Kellendonk C.. (), 'Striatal D2 receptors regulate dendritic morphology of medium spiny neurons via Kir2 channels. .' Journal of Neuroscience. 10.1523/JNEUROSCI.6056-11.2012.