Products: Primary Antibodies

Anti-Histone H2A.Z acetyl-Lys4/7/11 Antibody (N299/39)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-319	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-319	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

DB ELISA

Target Description:

H2A.Z Variant Histone 1 is encoded by the gene H2AZ1. H2AZ1 is a member of the histone H2A family. H2AZ1 is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic fatality. H2AZ1 is expressed in 239 organs with the highest levels in the myometrium. Diseases associated with H2AZ1 include testicular spermatocytic seminoma.

Immunogen:

Synthetic peptide amino acids 2-15 (AGG[acetyl-K]AG[acetyl-K]DSG[acetyl-K]AKT) of human Histone H2A.Z (also known as Histone H2A/Z, accession number P0C0S5)
Mouse: 100% identity (14/14 amino acids identical)
Human: 100% identity (14/14 amino acids identical)
93% identity with Histone H2A.V

Cross Reactivity:

Does not cross-react with unacetylated Histone H2A.Z Does not cross-react with Histones H2A acetyl-Lys4/8 or H4 acetyl-Lys4/7/11/15

Protein Name(s):

Histone H2A.Z (H2A/z)

Gene Name(s):

H2AFZ H2AZ

Antibody Registry ID:

Purified: AB_2315848
TC Supernatant: AB_2315847

Antibody Validation and Application Notes

This antibody has been validated using the following assays: