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Products: Primary Antibodies

Anti-HCN1 Antibody (N70/28)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-110
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$325.00
Brand
NeuroMab Logo
Catalog #
73-110
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$325.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

HCN1 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN1 is found in the hippocampus, neocortex, and cerebellar cortex of brain. Alterations in this protein have been implicated in the disease early infantile epileptic encephalopathy.

Immunogen:

Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, accession number Q9JKB0); Mouse: 96% identity (128/133 amino acids identical); Human: 90% identity (121/133 amino acids identical); Less than 50% identity with HCN2, HCN3 and HCN4

Cross Reactivity:

No cross-reactivity against HCN2

Protein Name(s):

HCN1

Gene Name(s):

Hcn1

Antibody Registry ID:

Purified: AB_2115181
TC Supernatant: AB_10672848


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.