Products: Primary Antibodies

Anti-HCN1 (N70/28)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-110
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-110
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Immunogen:

Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, accession number Q9JKB0)
Mouse: 96% identity (128/133 amino acids identical)
Human: 90% identity (121/133 amino acids identical)
<50% identity with HCN2, HCN3 and HCN4

Cross Reactivity:

No cross-reactivity against HCN2

Protein Name(s):

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Gene Name(s):

Hcn1

Antibody Registry ID:

Purified: AB_2115181
TC Supernatant: AB_10672848


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

100 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.