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Products: Primary Antibodies

Anti-GAD65/67 (L127/12)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
73-508
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$269.00
Catalog #
75-508
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$269.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

AT ICC IHC WB

Immunogen:

Fusion protein amino acids 1-594 (full-length) of human GAD67 (also known as Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1, accession number Q99259)

Mouse: 97% identity (577/594 amino acids identical)
Rat: 97% identity (577/594 amino acids identical)
100% identity (213/213 amino acids identical) with GAD25
>90% identity (366/391 amino acids identical, highest in N-terminus) with GAD44
>60% identity (375/569 amino acids identical, highest in C-terminus) with GAD2/GAD65/DCE2

Cross Reactivity:

Cross-reacts with GAD25, GAD44 and GAD65/GAD2

Protein Name(s):

GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD and DCE1

Gene Name(s):

GAD1, GAD, GAD67

Antibody Registry ID:

TC Supernatant: AB_2756510
Purified:


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

70 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.