Our Anti-GAD65/67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/12. It detects human, mouse, and rat GAD65/67, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
There are two forms of the enzyme glutamic-acid decarboxylase (GAD), a 65-kDa form (GAD65) and a 67-kDa form (GAD67) which are the result of the expression of different genes. Further splicing of GAD 67 produces one or two smaller variants, including GAD25 and GAD44. GAD67 and GAD65 are expressed in adult brain and the pancreas. The truncated forms GAD25 and GAD44 are expressed during embryonic development. Glutamic acid decarboxylase enzyme functions to catalyze the production of gamma-aminobutyric acid from L-glutamic acid.
Purified by Protein A chromatography
0.5 mg/mL
Monoclonal
L127/12
IgG2a
ICC, IHC
Mouse
GAD1, GAD, GAD67
70 kDa
Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2940693
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL550 Ex: 550 nm, Em: 575 nm
Cross-reacts with GAD25, GAD44 and GAD65/GAD2
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
