Products: Primary Antibodies

Anti-GABA(B)R2 (N81/2)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-124	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-124	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 861-912 of rat GABABR2 (also known as gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, Gb2, G-protein coupled receptor 51 or GPR51, accession number NP_113990) Mouse: 100% identity (52/52 amino acids identical) Human: 100% identity (52/52 amino acids identical <12% identity with GABABR1

Cross Reactivity:

No cross-reactivity against GABABR1

Protein Name(s):

Gamma-aminobutyric acid type B receptor subunit 2 (GABA-B receptor 2) (GABA-B-R2) (GABA-BR2) (GABABR2) (Gb2) (G-protein coupled receptor 51) (HG20)

Gene Name(s):

GABBR2 GPR51 GPRC3B

Antibody Registry ID:

Purified: AB_10672845
TC Supernatant: AB_10671704

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

105 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Workman ER, Haddick PC, Bush K, Dilly GA, Niere F, Zemelman BV, Raab-Graham KF.. (2015), 'Rapid antidepressants stimulate the decoupling of GABA(B) receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3η. .' Molecular Psychiatry. 10.1038/mp.2014.165.
Murthy R, Kim J, Sun X, Giger RJ, Fink DJ, Mata M.. (2013), 'Post-transcriptional regulation of GABAB receptor and GIRK1 channels by Nogo receptor 1. .' Molecular Brain. 10.1186/1756-6606-6-30.
Nguyen MV, Du F, Felice CA, Shan X, Nigam A, Mandel G, Robinson JK, Ballas N.. (2013), 'MeCP2 is critical for maintaining mature neuronal networks and global brain anatomy during late stages of postnatal brain development and in the mature adult brain. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1316-12.2012.
Maity B, Stewart A, Yang J, Loo L, Sheff D, Shepherd AJ, Mohapatra DP, Fisher RA.. (2012), 'Regulator of G protein signaling 6 (RGS6) protein ensures coordination of motor movement by modulating GABAB receptor signaling. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.297218.
Paris-Robidas S, Brochu E, Sintes M, Emond V, Bousquet M, Vandal M, Pilote M, Tremblay C, Di Paolo T, Rajput AH, Rajput A, Calon F.. (2012), 'Defective dentate nucleus GABA receptors in essential tremor. .' Brain. 10.1093/brain/awr301.
Lodewyks C, Rodriguez J, Yan J, Lerner B, Lipschitz J, Nfon C, Rempel JD, Uhanova J, Minuk GY.. (2011), 'GABA-B receptor activation inhibits the in vitro migration of malignant hepatocytes. .' Can The Journal of Physiology Pharmacol.. 10.1139/y11-031.
Pacey LK, Tharmalingam S, Hampson DR.. (2011), 'Subchronic administration and combination metabotropic glutamate and GABAB receptor drug therapy in fragile X syndrome. .' Journal of Pharmacology and Experimental Therapeutics. 10.1124/jpet.111.183327.
Broussard DM, Titley HK, Antflick J, Hampson DR.. (2011), 'Motor learning in the VOR: the cerebellar component. .' Experimental Brain Research. 10.1007/s00221-011-2589-z.
Pacey LK, Doss L, Cifelli C, van der Kooy D, Heximer SP, Hampson DR.. (2011), 'Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse. .' Molecular and Cellular Neuroscience. 10.1016/j.mcn.2010.12.005.
Nassirpour R, Bahima L, Lalive AL, Lüscher C, Luján R, Slesinger PA.. (2010), 'Morphine- and CaMKII-dependent enhancement of GIRK channel signaling in hippocampal neurons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2966-10.2010.