Products: Primary Antibodies

Anti-GABA(B)R2 Antibody (N81/2)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-124
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-124
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Target Description:

Gamma-aminobutyric acid type B receptor subunit 2 or GABA BR2 is encoded by the gene GABBR2. GABA BR2 is a subunit of the G-protein coupled receptor for the neurotransmitter GABA (gamma-aminobutyric acid). It will inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase. GABA BR2 is highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus at the cell junction, synapse, the postsynaptic cell membrane and cytoplasmic vesicle membrane. It is weakly expressed in heart, testis and skeletal muscle. Diseases associated with GABBR2 include Neurodevelopmental Disorder With Poor Language And Loss Of Hand Skills, Rett syndrome- and Early Infantile Epileptic Encephalopathy 59.

Immunogen:

Fusion protein amino acids 861-912 of rat GABABR2 (also known as gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, Gb2, G-protein coupled receptor 51 or GPR51, accession number NP_113990)
Mouse: 100% identity (52/52 amino acids identical)
Human: 100% identity (52/52 amino acids identical
Less than 12% identity with GABABR1

Cross Reactivity:

No cross-reactivity against GABABR1

Protein Name(s):

GABA(B)R2

Gene Name(s):

GABBR2 GPR51 GPRC3B

Antibody Registry ID:

Purified: AB_10672845
TC Supernatant: AB_10671704


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

105 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.