Bovine Chicken Human Mouse Rat
EM ICC IHC IP WB
Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: - rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol - immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex - recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472) Mouse: 100% identity (15/15 amino acids identical) Rat: 100% identity (15/15 amino acids identical) 86% identity (13/15 amino acids identical, QSVNDPSNMSLVKET) with GABA-A-R-Beta2 66% identity (10/15 amino acids identical, HSTNEPSNMSYVKET) with GABA-A-R-Beta1 Hybridoma kindly donated by Angel de Blas, University of Connecticut. This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.
Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1
Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)
TC Supernatant: AB_2315837
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.