Products: Primary Antibodies

Anti-Clcn5 (N406/47)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-400		 Option
Purified		 Volume
100 µL		 Concentration
1 mg/mL		 Price
$275.00
Catalog #
73-400		 Option
TC Supernatant		 Volume
5 mL		 Concentration
Lot dependent		 Price
$275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC

Immunogen:

Fusion protein amino acids 568-746 (C-terminus) of rat Clcn5 (also known as H(+)/Cl(-) exchange transporter 5, Chloride channel protein 5, Chloride transporter ClC-5, Clc5 and CLCK2, accession number P51796)
Mouse: 99% identity (178/179 amino acids identical)
Human: 98% identity (177/179 amino acids identical)
70% identity with Clcn3 and Clcn4

Cross Reactivity:

Does not cross-react with Clcn4

Protein Name(s):

H(+)/Cl(-) exchange transporter 5 (Chloride channel protein 5) (ClC-5) (Chloride transporter ClC-5)

Gene Name(s):

Clcn5

Antibody Registry ID:

Purified: AB_2491083
TC Supernatant: AB_2341103

Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.