Human Mouse Rat
ICC IHC WB
PSD-93/Chapsyn-110 (also known as Channel-associated protein of synapse-110, Postsynaptic density protein PSD-93, Disks large homolog 2 and Dlg2) is a member of the membrane-associated guanylate kinase (MAGUK) family. The protein contains a guanylate kinase-like domain, 3 PDZ domains and 1 SH3 domain. Chapsyn110 binds to NMDA receptor subunits and Shaker K+ channel subunits to aid in forming a multiprotein scaffold which clusters receptors, ion channels, cytoskeletal protiens and other cell signaling proteins. In this manner, it plays a key role in postsynaptic targeting. It is found in brain in neuronal cells and located at the cell synapse, postsynaptic cell membrane and postsynaptic density. Chapsyn110 has been implicated in chronic pain, bilateral retinoblastoma and schizophrenia.
Fusion protein amino acids 1-852 (full-length) of rat Chapsyn-110 (also known as Channel-associated protein of synapse-110, Postsynaptic density protein PSD-93, Disks large homolog 2, Dlg2 and Dlgh2, accession number Q63622)
Mouse: 99% identity (846/852 amino acids identical
Human: 94% identity (826/870 amino acids identical)
>60% identity with other MAGUK family members (SAP97/Dlg1, SAP102/Dlg3 and PSD-95/Dlg4)
Does not cross-react with SAP97, SAP102 or PSD-95
Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93)
TC Supernatant: AB_11000722
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Fasoli A1, Dang J1, Johnson JS1, Gouw AH1, Fogli Iseppe A1, Ishida AT1,2.. (2017), 'Somatic and neuritic spines on tyrosine hydroxylase-immunopositive cells of rat retina..' J Comp Neurol.. 10.1002/cne.24166.
- Liu Y1, Lee JW1, Ackerman SL2.. (2015), 'Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration..' J Neurosci.. 10.1523/JNEUROSCI.2757-14.2015.
- Fujikawa T1, Petralia RS2, Fitzgerald TS3, Wang YX4, Millis B5, Morgado-Díaz JA6, Kitamura K7, Kachar B5.. (2014), 'Localization of kainate receptors in inner and outer hair cell synapses..' Hear Res.. 10.1016/j.heares.2014.05.001.