Anti-Beta4-Spectrin Antibody (N393/2)

Name: Anti-Beta4-Spectrin Antibody
Brand: NeuroMab
SKU: 73-376
Price: 394.0 USD
Availability: InStock

Our mouse Beta4-spectrin monoclonal antibody from NeuroMab is produced in-house from clone N393/2. It detects human, mouse, and rat Beta4-spectrin and is TC supernatant. Works in WB, IHC, ICC, ELISA.

Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.

Adult rat cerebral cortex and hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment.

Confocal image stacks of representative Axo-axonic cells (AAC) (E) and Basket Cells (F). Center images illustrate two examples of axons from the cell in E showing close apposition for βIV-spectrin (Cat. 75-376, 1:500) labeling for axon initial segments (top), biocytin (middle) and merged (bottom). Arrowheads indicate regions of close apposition between βIV-spectrin labeling on axon initial segment of Granule Cell (GC) and biocytin filled axonal cartridge of AAC. CC-BY-4.0. PMID:37898313

Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 73-376

Volume: 5 mL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Beta4-spectrin

Scientific Background

Spectrin beta chain, non-erythrocytic 4, or ß4 spectrin, is a member of the spectrin family of cytoskeletal proteins. It is found in the cytoskeleton and cell cortex of cells in the brain and pancreatic islets. It is also expressed in skeletal muscule. ß4 spectrin functions as a scaffold protein that links the actin cytoskeleton to the plasma membrane. It is involved in cell shape and organization. Mutations in the ß4 spectrin gene are associated with congenital myopathy, and neurodevelopmental disorder with hypotonia, neuropathy and deafness (NEDHND).

Format

TC Supernatant

Concentration

Lot dependent

Clonality

Monoclonal

Clone

N393/2

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

SPTBN4 KIAA1642 SPTBN3

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315816

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Supernatant is harvested from hybridoma cells grown under standard cell culture conditions

Buffer

Supernatant is provided in cell culture medium with 0.1% sodium azide as anti-microbial

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with other Beta-spectrins

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Spectrin beta chain, non-erythrocytic 4 (Beta-IV spectrin) (Spectrin, non-erythroid beta chain 3)

UniProt ID

Q9H254

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Beta4-Spectrin Antibody (N393/2)

SKU: 73-376

Product Details

Bulk Order Anti-Beta4-Spectrin Antibody

Related Products

Recently Viewed