Anti-Beta4-Spectrin Antibody (N393/2)

2 Citations

Our Anti-Beta4-spectrin mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N393/2. It detects human, mouse, and rat Beta4-spectrin, and is TC supernatant. It is great for use in IHC, ICC, WB.

Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult rat cerebral cortex and hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment.
Confocal image stacks of representative Axo-axonic cells (AAC) (E) and Basket Cells (F). Center images illustrate two examples of axons from the cell in E showing close apposition for βIV-spectrin (cat. 75-376, 1:500) labeling for axon initial segments (top), biocytin (middle) and merged (bottom). Arrowheads indicate regions of close apposition between βIV-spectrin labeling on axon initial segment of Granule Cell (GC) and biocytin filled axonal cartridge of AAC. CC-BY-4.0. PMID:37898313
Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.
Roll over image to zoom in Click on image to zoom
Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.
Adult rat brain membrane immunoblot.
Adult rat cerebral cortex and hippocampus immunohistochemistry with antigen retrieval via pepsin pretreatment.
Confocal image stacks of representative Axo-axonic cells (AAC) (E) and Basket Cells (F). Center images illustrate two examples of axons from the cell in E showing close apposition for βIV-spectrin (cat. 75-376, 1:500) labeling for axon initial segments (top), biocytin (middle) and merged (bottom). Arrowheads indicate regions of close apposition between βIV-spectrin labeling on axon initial segment of Granule Cell (GC) and biocytin filled axonal cartridge of AAC. CC-BY-4.0. PMID:37898313

Human, Mouse, Rat
ICC, IHC, WB
Mouse

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Data Sheet

SKU: 73-376

Volume: 5 mL
Price:
$385.00
Ships: 1-2 business days

Product Specific References for Applications and Species

  • Immunocytochemistry: Rat
  • Immunohistochemistry: Mouse
Immunocytochemistry: Rat
PMID Dilution Publication
29395909not listedBerger, S.L., et al. 2018. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Neuron, 555-570.
Immunohistochemistry: Mouse
PMID Dilution Publication
378983131:500Proddutur, A, et al. 2023. Reclusive chandeliers: Functional isolation of dentate axo-axonic cells after experimental status epilepticus. Progress in Neurobiology, 102542.

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