Products: Primary Antibodies

Anti-Ataxin-1, WT (N65/37)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-102	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-102	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Synthetic peptide amino acids 746-761 (RKRRRWSAPETRKLEK) of mouse ataxin-1 (also known as spinocerebellar ataxia type 1 protein homolog, accession number P54254)
Rat: 93% identity (15/16 amino acids identical)
Human: 87% identity (14/16 amino acids identical)

Cross Reactivity:

Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot (right)

Protein Name(s):

Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)

Gene Name(s):

Atxn1 Sca1

Antibody Registry ID:

Purified: AB_10675132
TC Supernatant: AB_10675131

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

85 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Citations and References

Ruan K,, Zhu Y,, Li C,, Brazill JM,, Zhai RG,,. (2015), 'Alternative splicing of Drosophila Nmnat functions as a switch to enhance neuroprotection under stress. .' Nature Communications. 10.1038/ncomms10057.
Menon RP, Nethisinghe S, Faggiano S, Vannocci T, Rezaei H, Pemble S, Sweeney MG, Wood NW, Davis MB, Pastore A, Giunti P.. (2013), 'The role of interruptions in polyQ in the pathology of SCA1. .' PLOS Genetics. 10.1371/journal.pgen.1003648.