Human Mouse Rat
Synthetic peptide amino acids 746-761 (RKRRRWSAPETRKLEK) of mouse ataxin-1 (also known as spinocerebellar ataxia type 1 protein homolog, accession number P54254)
Rat: 93% identity (15/16 amino acids identical)
Human: 87% identity (14/16 amino acids identical)
Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot (right)
Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)
TC Supernatant: AB_10675131
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Citations and References
- Ruan K,, Zhu Y,, Li C,, Brazill JM,, Zhai RG,,. (2015), 'Alternative splicing of Drosophila Nmnat functions as a switch to enhance neuroprotection under stress. .' Nature Communications. 10.1038/ncomms10057. " "
- Menon RP, Nethisinghe S, Faggiano S, Vannocci T, Rezaei H, Pemble S, Sweeney MG, Wood NW, Davis MB, Pastore A, Giunti P.. (2013), 'The role of interruptions in polyQ in the pathology of SCA1. .' PLOS Genetics. 10.1371/journal.pgen.1003648. "