Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling #5843, magenta) and DAPI (blue). Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford)..
Bulk Order Anti-VGAT Antibody
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
ICC, IHC, WB
Slc32a1 Vgat Viaat
Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Supernatant is harvested from hybridoma cells grown under standard cell culture conditions
Supernatant is provided in cell culture medium with 0.1% sodium azide as anti-microbial
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from date of receipt
Shipped on ice packs
Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)