Anti-VGAT Antibody (L118/66)

Name: Anti-VGAT Antibody
Brand: NeuroMab
SKU: 73-478
Price: 337.0 USD
Availability: InStock

Our mouse VGAT monoclonal antibody from NeuroMab is produced in-house from clone L118/66. It detects mouse and rat VGAT and is TC supernatant. Works in WB, IHC, ICC, ELISA, AT.

Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford).

Adult rat brain hippocampus immunohistochemistry.

Adult rat brain cortex immunohistochemistry.

Array tomography immunofluorescence of a single 70 nm section (left) and volume reconstruction of 8 serial sections (right) of LRWhite-embedded adult mouse cerebellum with L118/66 (VGAT, green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). Purkinje cells (PC) have a low level of VGAT immunoreactivity, and numerous inhibitory synaptic varicosities in molecular (M) and granule (G) layers are colabeled with VGAT and GAD2. Images courtesy of Kristina Micheva (Stanford).

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 73-478

Volume: 5 mL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGAT

Scientific Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Format

TC Supernatant

Concentration

Lot dependent

Clonality

Monoclonal

Clone

L118/66

Isotype

IgG2a

Applications

AT, ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Slc32a1 Vgat Viaat

Molecular Weight

50 kDa

Immunogen

Fusion protein amino acids 1-133 (cytoplasmic N-terminus) of mouse VGAT (accession number O35633) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2650550

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Supernatant is harvested from hybridoma cells grown under standard cell culture conditions

Buffer

Supernatant is provided in cell culture medium with 0.1% sodium azide as anti-microbial

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular inhibitory amino acid transporter (GABA and glycine transporter) (Solute carrier family 32 member 1) (Vesicular GABA transporter) (mVGAT) (mVIAAT)

UniProt ID

O35633

Shipping

Shipped on ice packs

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Anti-VGAT Antibody (L118/66)

SKU: 73-478

Product Details

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