Anti-TMEM240 Recombinant Chicken Chimeric mAb (N498/28)

Our chicken Anti-TMEM240 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N498/28. It detects human, mouse, and rat TMEM240 and is purified by affinity chromatography. It works in ELISA, ICC, IHC, WB.

Immunohistochemistry for detection of TMEM240 in adult rat hippocampus using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-TMEM240 antibody (Cat No. 78-533) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Western blot detection of TMEM240 using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). 4-20% Tris-Glycine Gel was loaded with rat brain membrane fraction. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-TMEM240 antibody was incubated with sample at a concentration of 1 ug/ml and then detected via chemiluminescence (3.5 min) using a Goat Anti-Chicken H+L, HRP conjugated secondary antibody. Anti-TMEM240 recognizes endogenous TMEM240 in the rat brain membrane fraction at the expected molecular weight of ~20 kDa.
Immunohistochemistry for detection of TMEM240 in adult rat cerebellum using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-TMEM240 antibody (Cat No. 78-533) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-TMEM240 preferentially stains the Purkinje cells in the cerebellum as expected.
Immunohistochemistry for detection of TMEM240 in adult rat cerebellum using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-TMEM240 antibody (Cat No. 78-533) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-TMEM240 preferentially stains the Purkinje cells in the cerebellum as expected.
Immunohistochemistry for detection of TMEM240 in adult rat hippocampus using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-TMEM240 antibody (Cat No. 78-533) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
Immunohistochemistry for detection of TMEM240 in adult rat hippocampus using Aves Labs’ anti-TMEM240 recombinant chicken monoclonal antibody [N498/28] (Cat No. 78-533). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-TMEM240 antibody (Cat No. 78-533) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-chicken IgY CF488 secondary antibody (Cat No. 80-1001-488) (green). DAPI nuclear stain (blue) shows cell nuclei.
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Human, Mouse, Rat
ELISA, ICC, IHC, WB
Chicken
490
550
594
650
Carrier-Free

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SKU: 78-533

Volume: 100 µL
Price:
$481.00
Ships: 1-2 business days

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