Anti-Shank3 Antibody FL594 Conjugate (N367/62)

Name: Anti-Shank3 Antibody FL594 Conjugate
Brand: NeuroMab
SKU: 75-344-FL594
Price: 543.0 USD
Availability: InStock

Our mouse Shank3 FL594 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N367/62. It detects mouse and rat Shank3 and is purified by Protein A chromatography. KO validated. Works in IHC, ICC.

Anti-Shank3 Antibody FL594 Conjugate

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Species Reactivity

Mouse, Rat

Applications

ICC, IHC

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-344-FL594

Volume: 200 µL

Variant

Price:

$543.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Shank3

Scientific Background

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Monoclonal

Clone

N367/62

Isotype

IgG2a

Applications

ICC, IHC

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (accession number Q9JLU4) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2940170

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL594 Ex: 594 nm, Em: 615 nm

Specificity

Does not cross-react with Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

Prosap2; SH3 and multiple ankyrin repeat domains protein 3; SH3/ankyrin domain gene 3; Shank3; SPANK-2; Proline-rich synapse-associated protein 2; Shank postsynaptic density protein 3a

UniProt ID

Q9JLU4
UniProt Summary: Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

NCBI Gene ID

59312

Shipping

Shipped on ice packs

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Anti-Shank3 Antibody FL594 Conjugate (N367/62)

SKU: 75-344-FL594

Product Details

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