Anti-Shank3 Antibody (N367/62)

Name: Anti-Shank3 Antibody
Brand: NeuroMab
SKU: 75-344
Price: 394.0 USD
Availability: InStock

Our mouse Shank3 monoclonal antibody from NeuroMab is produced in-house from clone N367/62. It detects mouse and rat Shank3 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Distribution of SHANK3 immuno-positive cells in different regions of the adult mouse cerebral cortex. N, O: High-magnification views of SHANK3-positive cells in the RSPV region (N) and hippocampal region (O) as shown in image M. Green, SHANK3-positive cells (Cat. 75-344, 1:200); blue, cell nuclei. Scale bars, 300 μm (M); 100 μm (N, O). Image from publication, CC-BY-4.0. PMID: 39693031.

Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/62 (left) or N23B/49 (right) TC supe.

Immunoblot against adult rat brain membranes (RBM) and membranes from Shank3 wild-type (WT) and knockout (KO) mice probed with N367/62 (left) or K58/35 (right) TC supe. Mouse brains courtesy of Yuan Mei, Holly Robertson and Guoping Feng (MIT).

Western Blots and analysis of SHANK3 (Cat. 75-344, 1:200) protein expression levels at different developmental stages in the mouse cerebral cortex. (n = 3 mice/group, ****P PMID: 39693031

Distribution of SHANK3 immuno-positive cells in different regions of the adult mouse cerebral cortex. N, O: High-magnification views of SHANK3-positive cells in the RSPV region (N) and hippocampal region (O) as shown in image M. Green, SHANK3-positive cells (Cat. 75-344, 1:200); blue, cell nuclei. Scale bars, 300 μm (M); 100 μm (N, O). Image from publication, CC-BY-4.0. PMID: 39693031.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-344

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Shank3

Scientific Background

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N367/62

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Immunogen

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (accession number Q9JLU4) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315921

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Prosap2; SH3 and multiple ankyrin repeat domains protein 3; SH3/ankyrin domain gene 3; Shank3; SPANK-2; Proline-rich synapse-associated protein 2; Shank postsynaptic density protein 3a

UniProt ID

Q9JLU4
UniProt Summary: Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

NCBI Gene ID

59312

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Anti-Shank3 Antibody (N367/62)

SKU: 75-344

Product Details

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