Name: Anti-Shank3 Antibody
Brand: NeuroMab
SKU: 75-344
Price: 335.0 USD
Availability: InStock

Clonality

Monoclonal

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Go to Full Product Details

Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/62 (left) or N23B/49 (right) TC supe.

Immunoblot against adult rat brain membranes (RBM) and membranes from Shank3 wild-type (WT) and knockout (KO) mice probed with N367/62 (left) or K58/35 (right) TC supe. Mouse brains courtesy of Yuan Mei, Holly Robertson and Guoping Feng (MIT).

Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Product Details

Target

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Target Description

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N367/62

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Antigen

Fusion protein amino acids 538-626 (SH3 domain) of rat Shank3 (also known as SH3 and multiple ankyrin repeat domains protein 3, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SPANK-2 and Kiaa1650, accession number Q9JLU4); Mouse: 100% identity (89/89 amino acids identical); Human: 97% identity (87/89 amino acids identical); ~70% identity with Shank1 and Shank2

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315921

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Conjugate

Unconjugated

Specificity

Does not cross-react with Shank1 or Shank2

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

UniProt Details

UniProt (Immunogen Species): Q9JLU4

Citations (5)

Harony-Nicolas H1,2, Kay M3, Hoffmann JD1,4, Klein ME5, Bozdagi-Gunal O1,2, Riad M1,2, Daskalakis NP2, Sonar S1,2, Castillo PE5, Hof PR1,4,6, Shapiro ML4,6, Baxter MG4,6, Wagner S3, Buxbaum JD1,2,4,6,7,8. (2017), 'Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat..' Elife. 10.7554/eLife.18904.
Zhou Y1, Kaiser T2, Monteiro P3, Zhang X2, Van der Goes MS2, Wang D2, Barak B2, Zeng M4, Li C5, Lu C5, Wells M6, Amaya A7, Nguyen S7, Lewis M7, Sanjana N5, Zhou Y8, Zhang M9, Zhang F10, Fu Z5, Feng G11. (2016), 'Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects..' Neuron. 10.1016/j.neuron.2015.11.023.
MacGillavry HD1,2,3, Kerr JM1,2, Kassner J1, Frost NA1,2,4, Blanpied TA1,2. (2016), 'Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses..' Eur J Neurosci. 10.1111/ejn.13129.
Berg JM1, Lee C2, Chen L2, Galvan L3, Cepeda C3, Chen JY3, Peñagarikano O2, Stein JL2, Li A2, Oguro-Ando A2, Miller JA2, Vashisht AA4, Starks ME2, Kite EP2, Tam E2, Gdalyahu A5, Al-Sharif NB3, Burkett ZD6, White SA6, Fears SC3, Levine MS3, Wohlschlegel JA4, Geschwind DH7. (2015), 'JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse..' Neuron. 10.1016/j.neuron.2015.10.031.
Duffney LJ1, Wei J, Cheng J, Liu W, Smith KR, Kittler JT, Yan Z. (2013), 'Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism..' J Neurosci. 10.1523/JNEUROSCI.1175-13.2013.

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Anti-Shank3 Antibody (N367/62)

Anti-Shank3 Antibody
SKU: 75-344

Product Details

Citations (5)

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Anti-Shank3 Antibody (N367/62)

Anti-Shank3 Antibody SKU: 75-344

Product Details

Citations (5)

Anti-Shank3 Antibody
SKU: 75-344