Anti-Ryanodine Receptor Type 2 Recombinant Chicken Chimeric mAb Biotin Conjugate (N501/49)

Name: Anti-Ryanodine Receptor Type 2 Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-534-BTN
Price: 562.0 USD
Availability: InStock

Our chicken RyR2 biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone N501/49. It detects human, mouse, and rat RyR2 and is purified by affinity chromatography. Works in ELISA, ICC, IHC, WB.

Anti-Ryanodine Receptor Type 2 Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-534-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

RyR2

Scientific Background

The Ryanodine Receptor 2 (RyR2) is important in cardiac physiology, historically identified through its high affinity for the plant alkaloid ryanodine. First cloned and characterized in the late 1980s, it is key in understanding of how the heart beats at a molecular level. Unlike its skeletal muscle counterpart (RyR1), RyR2 is the primary calcium release channel located on the sarcoplasmic reticulum (SR) of cardiac muscle cells. Biologically, RyR2 is the gatekeeper of excitation-contraction coupling. When an action potential triggers a small influx of calcium through L-type channels, RyR2 senses this and responds by releasing a large "spark" of calcium from internal stores into the cytosol—a process known as calcium-induced calcium release (CICR). This sudden surge in Ca2+ concentration allows the heart muscle to contract. Because of its large size and complex regulatory domains, RyR2 is a hotspot for genetic mutations; even slight "leaks" in the channel during the heart's resting phase can lead to fatal arrhythmias, such as Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT), or contribute to chronic heart failure.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N501/49

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Ryr2

Molecular Weight

564 kDa

Immunogen

Recombinant Protein Amino acids 2672-2835 (cytoplasmic) of mouse Ryanodine Receptor Type 2 (also known as RyR2, Ryr2, accession number E9Q401) produced recombinantly in E. Coli.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N501/49 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N501/49 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:100-1:500
ICC: 1:100-1:500

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

9330127I20Rik; RyR2; ryanodine receptor 2; calcium release channel; cardiac muscle ryanodine receptor; ryanodine receptor type 2; type 2 ryanodine receptor; RYR-2

UniProt ID

E9Q401
UniProt Summary: Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) cytosolic levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.

NCBI Gene ID

20191

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Anti-Ryanodine Receptor Type 2 Recombinant Chicken Chimeric mAb Biotin Conjugate (N501/49)

SKU: 78-534-BTN

Product Details

Bulk Order Anti-Ryanodine Receptor Type 2 Recombinant Chicken Chimeric mAb Biotin Conjugate

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