Immunoblot against adult rat brain membrane (RBM) or low- speed pellet (RBLSP) fractions denatured at 95oC for 5 min or 37oC for 15 min and probed with N253/32 (left) and K58/35 (right) TC supe.
Bulk Order Anti-Notch1 Antibody
Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT); Notch 1 intracellular domain (NICD)]
Notch Receptor 1 is encoded by the gene NOTCH1. NOTCH1 is a member of the NOTCH family. NOTCH1 is involved in processes related to cell fate specification, differentiation, proliferation, and survival. NOTCH1 is highly expressed in the brain, lungs, and thymus. Diseases associated with NOTCH1 include Aortic Valve Disease 1 and Adams-Oliver Syndrome 5.
>270 kDa, ~120 kDa and smaller fragments (due to proteolysis)
Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (also known as Neurogenic locus notch homolog protein 1, hN1, Translocationassociated notch protein, TAN-1, Motch A, mT14, p300 and Motch, accession number Q01705)
Rat: 93% identity (185/197 amino acids identical)
Human: 84% identity (166/197 amino acids identical)
~40% identity with Notch2, Notch3 and Notch4
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.