Anti-Neurotrophin-4/5 (NT-4/5) Antibody

Name: Anti-Neurotrophin-4/5 (NT-4/5) Antibody
Brand: Biosensis
SKU: S-058-100
Price: 327.0 USD
Availability: InStock

Our Anti-Neurotrophin-4/5 (NT-4/5) sheep polyclonal primary antibody detects human, mouse, non-human primate, and rat Neurotrophin-4/5 (NT-4/5), and is whole serum. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

The effect of anti-NT4 antibody on the neurite outgrowth of embryonic dorsal root ganglion promoted by NT4. The ED50 is approximately 3.6 µg/mL.

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Species Reactivity

Human, Monkey, Mouse, Rat

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

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Data Sheet

SKU: S-058-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Neurotrophin-4/5 (NT-4/5)

Scientific Background

FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC, Neutralize, WB

Host Species

Sheep

Immunogen

Recombinant human NT4

Immunogen Species

Human

Species Reactivity

Human, Monkey, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:500-1:2000
IHC: 1:500-1:2000
ELISA: 1:500-1:2000

Application Details

1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control.

Conjugate

Unconjugated

Specificity

Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4

UniProt ID

P34130

Shipping

25°C (ambient)

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Anti-Neurotrophin-4/5 (NT-4/5) Antibody

SKU: S-058-100

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