Anti-Nav1.8 Na+ Channel Antibody (N134/12)

Name: Anti-Nav1.8 Na+ Channel Antibody
Brand: NeuroMab
SKU: 75-166-020
Price: 394.0 USD
Availability: InStock

Our mouse Nav1.8 Na+ channel monoclonal antibody from NeuroMab is produced in-house from clone N134/12. It detects human, mouse, and rat Nav1.8 Na+ channel and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.

Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.

Immunofluorescence staining of cultured dorsal root ganglia neurons from Nav1.8 WT and KO mice with N134/12 TC supe. Image courtesy of Dr. Joel Black, Dr. Sulayman Dib-Hajj and Dr. Steve Waxman, Yale University.

Immunofluorescence staining of rat dorsal root ganglia cryosections. N134/12 TC supe = green, neurofilament = red. Image courtesy of Dr. Joel Black, Dr. Sulayman Dib-Hajj and Dr. Steve Waxman, Yale University.

Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-166-020

Volume: 20 µL

Variant

Price:

$142.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nav1.8 Na+ channel

Scientific Background

NAV1.8 is a member of the voltage-gated sodium channel family, which function in producing and propagating action potentials. Sodium channels are composed of a large alpha subunit that may be associated with one or more smaller beta subunits. Nav 1.8 is an integral membrane glycoprotein that is responsible for the initial rising phase of action in most excitable cells. Nav 1.8 is expressed in neurons in the dorsal root ganglion (DRG). and appears to have a role in pain pathways.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N134/12

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Scn10a Sns

Molecular Weight

220 kDa

Immunogen

Fusion protein amino acids 1724-1956 (cytoplasmic C-terminus) of rat Nav1.8 (accession number Q62968) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2183861

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.93

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

Does not react with Nav1.7

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Na(V1.8; Sodium channel protein type 10 subunit alpha; PN3; Peripheral nerve sodium channel 3; Sensory neuron sodium channel; Sodium channel protein type X subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.8; sodium channel type X alpha polypeptide

UniProt ID

Q62968
UniProt Summary: Tetrodotoxin-resistant channel that mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Plays a role in neuropathic pain mechanisms.

NCBI Gene ID

29571

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Anti-Nav1.8 Na+ Channel Antibody (N134/12)

SKU: 75-166-020

Product Details

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