Anti-Kir2.3 K+ Channel Antibody (N25/35)

Name: Anti-Kir2.3 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-069
Price: 319.0 USD
Availability: InStock

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Our Anti-Kir2.3 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N25/35. It detects human, mouse, and rat Kir2.3 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.$

Adult rat brain immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-069

Volume: 100 µL

Variant

Price:

$319

Quantity:

Product Details

Target

Kir2.3 K+ channel

Target Description

Inward rectifier potassium channel 4 or Kir2.3 is encoded by the gene KCNJ4. Kir2.3 is a member of the inward rectifier potassium ion channel family and plays a role in excitability of muscle cells and neurons. Kir2.3 is an integral membrane protein that is found expressed in heart, skeletal muscle, and several different brain regions including the hippocampus. Diseases associated with KCNJ4 include Long Qt Syndrome 9 and Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N25/35

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

KCNJ4 IRK3

Molecular Weight

45 kDa

Antigen

Fusion protein amino acids 390-445 of human Kir2.3 (accession number NP_032453) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2130742

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

No cross-reactivity against Kir2.1 or Kir2.2

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Inward rectifier potassium channel 4 (HIRK2) (HRK1) (Hippocampal inward rectifier) (HIR) (Inward rectifier K(+) channel Kir2.3) (IRK-3) (Potassium channel, inwardly rectifying subfamily J member 4)

UniProt Details

UniProt (Human): P48050
UniProt (Immunogen Species): P48050

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PLA: Rat
Western Blot: Human | Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28452394	not listed	Frank, R., et al. 2017. Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes. Journal of neurochemistry, 504-511.

PLA: Rat
PMID	Dilution	Publication
36060074	1:200	Xu, Y., et al. 2022. Hypoxia Inhibits the Cardiac I Current Through SUMO Targeting Kir2.1 Activation by PIP2. iScience, 104969.

Western Blot: Human
PMID	Dilution	Publication
24427266	1:2000	Sivagangabalan, G., et al. 2014. Regional ion channel gene expression heterogeneity and ventricular fibrillation dynamics in human hearts. PloS one, e82179.

Western Blot: Mouse
PMID	Dilution	Publication
28452394	not listed	Frank, R., et al. 2017. Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes. Journal of neurochemistry, 504-511.
22396414	1:100	Cavorla, M., et al. 2012. Striatal D2 receptors regulate dendritic morphology of medium spiny neurons via Kir2 channels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2398-2409.

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Anti-Kir2.3 K+ Channel Antibody (N25/35)

SKU: 75-069

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Kir2.3 K+ Channel Antibody

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